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KMID : 0386319800130010049
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Korean Leprosy Bulletin
1980 Volume.13 No. 1 p.49 ~ p.55
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A Comparative Study on Various Staining Methods for Mycobacterium Leprae in Skin Smears of Leprosy Patients
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Abstract
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Two staining methods, the carbol fuchsin with acetic acid differentiation and the
periodic acid-carbol pararosanaline, were used for demonstrating leprosy bacilli in skin
smears of leprosy patients. The skin smears from 308 long-treated patients with
lepromatous, borderline and tuberculoid leprosy were stained with periodic acid-carbol
pararosaniline.
There were no significant differences in BI and MI determinations than with classic
carbol fuchsin staining, however slightly higher BI and MI results were observed than
with latter staining.
With the former stain, leprosy bacilli were found in 8 of 234 skin smears in which no
bacilli could be seen by the batter stain.
It is considered that under the action of antileprosy drugs some leprosy bacilli may
lose their acid-fastness and become chromophobic and these chromophobic bacilli can be
restored their acid-fastness in their staining with periodic acid pretreatment as reported
by Dr. Harada etc.
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KEYWORD
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